Haemophilus and Other Fastidious Gram-Negative Bacilli, Atlas of Haemophilus and Other Fastidious Gram-Negative Bacilli, SUBCLINICAL ATLAS, MICROBIOLOGY ATLAS, tuyenlab.net, atlas in medical
1. Haemophilus, HACEK Group, and Similar Microorganisms
|Fig 1. Prevalence of gram-negative bacilli isolated from cultures in a large|
tertiary hospital. Data on Pasteurella, Brucella, Legionella, and Bordetella are not included.
|Fig 2. Haemophilus influenzae satellitism around|
and between the large, white, hemolytic staphylococci. The
small, gray glistening colony is H. influenzae (arrow).
|Fig 3. Lesions of chancroid on the penis, showing|
draining bubo (arrow) in the adjacent groin area. Chancroid
is caused by Haemophilus ducreyi.
|Fig 4. Direct smear of Haemophilus influenzae|
in cerebrospinal fluid in a case of meningitis. Note the
intracellular and extracellular, gram-negative coccobacilli.
|Fig 5. Gram stain of a Haemophilus influenzae colony.|
Note the slightly more elongated bacilli.
|Fig 6. Example of Haemophilus influenzae growingon chocolate agar. |
Notice the tan mucoid colonies characteristic of encapsulated strains
|Fig 7. This organism would be identified as Haemophilus influenzae |
because it requires both X and V factors.
|Fig 8. This organism requires V factor only would beidentified as |
|Fig 9. This organism is positive for X factor only.|
The probable species is Aggregatibacter aphrophilus because
this species can appear to be hemin dependent on initial isolation.
|Fig 10. Under ultraviolet light, the organism on the bottom is exhibiting a positive porphyrin reaction. The organism on the top is porphyrin negative|
|Fig 11. An Aggregatibacter aphrophilus isolate that is not X factor |
dependent and is growing over the entire
surface of a trypticase soy agar plate.
|Fig 12. Aggregatibacter aphrophilus growing onsheep blood agar.|
|Fig 13. Gram stain, microscopic morphology of Aggregatibacter aphrophilus (1000×).|
|Fig 14. Gram stain morphology of Aggregatibacter aphrophilus|
|Fig 16. The 48-hour growth of colonies of Cardiobacterium hominis on sheep blood agar.|
|Fig 17. Gram stain of Cardiobacterium hominisshowing typical “rosettes” (1000×).|
|Fig 18. Growth of Eikenella corrodens on chocolate agar.|
|Fig 19. Gram-stain morphology of Eikenella corrodens (1000×)|
|Fig 20. Gram stain of Kingella kingae illustrating the plump bacilli in chains. Compare with the other members of the HACEK group (1000×).|
|Fig 21. Growth of Capnocytophaga organisms onchocolate agar. |
Notice the spreading away from the center
of the colony. Compare this growth with Eikenella
|Fig 22. Gram stain of Capnocytophaga organisms(1000×). |
Notice the thin fusiform bacilli.
|Fig 23. Advanced periodontitis. Periodontitis is the inflammation of the periodontium caused by a complex reaction initiated when subgingival plaque bacteria are in|
close contact with the epithelium of the gingival sulcus
|Fig 24. Pasteurella multocida growing on sheep blood agar and chocolate agar.|
The MacConkey agar plate is negative growth.
|Fig 25. Brucella melitensis colonies on sheep blood agar appear smooth, raised, and translucent.|
|Fig 26. Francisella tularensis colonies grown onchocolate agar. |
Gray-white, raised colonies with a smooth
appearance are visible following 72 hours of incubation.
|Fig 27. Gram stain of specimen demonstratingintracellular and |
extracellular Legionella pneumophila(1000×).
|Fig 30. Schema for identification of Legionella organisms. BCYE, Bufferedcharcoal yeast extract; LRT, lower respiratory tract; DFA, direct fluorescent antibody.|
*Biohazard precautions; consider organisms such as Francisella spp.
|Fig 31. A, Legionella pneumophila in specimen smear stained by directfluorescent antibody (DFA) technique (450×). B, Legionella pneumophila in specimen|
smear stained by DFA technique (1000×). Note intense peripheral staining of the
|Fig 32. Five-day-old colonies of Bordetella pertussison charcoal–horse blood agar (incident light from lower right corner).|
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