Direct examination in Parasitic infections, Acanthamoeba spp, Toxoplasma gondii, Pneumocystis jiroveci, Enterocytozoon bieneusi, Cyclospora, Cryptosporidium parvum,Strongyloides stercoralis, Phthirus pubis, Trichinella spiralis, atlas in medical, tuyenlab.net, Atlas of Direct examination in Parasitic infections
|Fig 1. Cornea, scraping, Wright-Giemsa stain, light|
microscopy, HPV. Purulence, none. Local materials,
moderate. Parasitic precyst (13 µm). Morphology consistent
with Acanthamoeba spp.
|Fig 2. Cornea, scraping from Plate 91, calcofluor white|
stain, fluorescence microscopy, MPV. Polyhedral parasitic cyst
(13 µm). Morphology consistent with Acanthamoeba cyst.
Impression: Acanthamoeba keratitis.
|Fig 3. BAL, cytocentrifuge preparation, Wright-Giemsa|
stain, light microscopy, HPV. Purulence, light. Local materials,
light. Amorphous debris light. Crescent-shaped cells with
central nucleus (see Fig 4).
|Fig 4. BAL, cytocentrifuge preparation, acridine orange|
stain, fluorescence microscopy, HPV. Crescent-shaped cells
composed of RNA. Morphology consistent with trophozoites
(tachyzoites) of Toxoplasma gondii. Impression:
|Fig 6. BAL, cytocentrifuge preparation, calcofluor white|
stain, fluorescence microscopy, HPV. Fluorescent cysts with
coccoid bodies. Morphology consistent with P. jiroveci
(carinii). Impression: pneumocystosis.
|Fig 7. Diarrheic stool, smear, modified Weber stain,|
light microscopy, 2000× oil. Purulence, none. Parasite spores,
small (1.5 × 0.9 µm). Morphology consistent with
enterocytozoon (arrow 1) (see Fig 8). Compare with size
of the yeast (arrow 2).
|Fig 8. Diarrheic stool from concentration, transmission|
electron microscopy, 57,000×. Endospore layer and polar
tubes present. Morphology consistent with Enterocytozoon
bieneusi. Impression: intestinal microsporidiosis.
|Fig 10. Watery, frothy diarrheic stool from Plate 99,|
smear, acid-fast stain, HPV. Acid-fast oocysts (4 to 6 µm).
Sporulated oocysts containing four sporozoites. Morphology
consistent with Cryptosporidium parvum. Impression:
|Fig 12. Aspirated sputum, Gram stain, light microscopy,|
LPV. Purulence, none. Local materials, moderate. Mucus
present. Coiled nematode larvae. Morphology consistent
with Strongyloides stercoralis. Impression: Strongyloides
|Fig 13. Parasite, pubic hair from surgical patient,|
unstained mount, light microscopy, LPV. Three pairs of legs
are identified with characteristic claws at tips. Morphology
consistent with Phthirus pubis (crab louse). Impression: louse
|Fig 14. Muscle tissue, directly viewed. Encysted calcified|
larvae. Morphology consistent with Trichinella spiralis.