Atlas of Anaerobes of Clinical Importance, Anaerobes of Clinical Importance, MICROBIOLOGY ATLAS, SUBCLINICAL ATLAS, atlas in microbiology, atlas in medical, tuyenlab.net, Connie R. Mahon, Donald C. Lehman and George Manuselis,
|Fig 1. Classification of some clinically encountered clostridia by endospore|
location. A, Gram-stained appearance of terminal spores of Clostridium tetani.
B, Gram-stained appearance of subterminal spores of Clostridium sordellii.
|Fig 2. Gram-stained appearance of Actinomyces|
israelii, illustrating the term Actinomyces-like.
|Fig 3. Anaerobic specimen collection and transport|
systems. Left: BBL Port-A-Cul with prereduced gel. Right: ESwab with
prereduced liquid Amies
|Fig 4. Prereduced, anaerobically sterilized (PRAS)|
plated media. PRAS plated media are manufactured,
packaged, shipped, and stored under anaerobic conditions.
|Fig 6. “Glove box” type of anaerobic chamber. The flexible, clear|
vinyl chambers are available in three lengths (36, 59, and 78
inches), including one fitted with two pairs of gloves so that
two microbiologists can use the chamber simultaneously.
|Fig 7. “Gloveless” type of anaerobic chamber with|
dissecting microscope attachment. This stainless steel and
Plexiglas chamber is manufactured by Anaerobe Systems.
|Fig 8. Anaerobic jars. This photograph depicts two|
of the many different types of anaerobic jars that are
available commercially. As can be seen in this photograph,
the jars can also be used to culture microaerophilic
|Fig 11. Schematic diagram for the initial identification of anaerobic isolates|
based on Gram-stain morphology. Not all Clostridium spp. readily sporulate in clinical
specimens or in culture.
|Fig 12. Examples of fluorescence observed with|
long-wave ultraviolet light. A, Brick-red fluorescence
observed with Porphyromonas asaccharolytica. B, Chartreuse
fluorescence of Fusobacterium nucleatum.
|Fig 14. Typical special-potency antimicrobial disk|
results for Clostridium ramosum: susceptible to vancomycin
(left) and kanamycin (right), and resistant to colistin (center
|Fig 15. Positive lecithinase reaction on egg-yolk|
agar. The reaction occurs within the agar. Clostridium
perfringens is shown here.
|Fig 16. Positive lipase reaction on egg-yolk agar.|
The reaction occurs on the surface of colonies and the
surrounding medium. A positive reaction by Fusobacterium
necrophorum is shown here.
|Fig 17. Double zone of hemolysis produced by|
Clostridium perfringens: inner zone of complete β-hemolysis
and outer zone of partial β-hemolysis.
|Fig 18. Appearance of Bacteroides fragilis on a|
kanamycin-vancomycin-laked blood agar (left) and
Bacteroides bile-esculin (BBE) agar biplate (right). Browning
of the BBE medium is the result of esculin hydrolysis.
|Fig 19. Appearance of Bilophila wadsworthia on|
BBE agar. Note the “fish-eye” appearance of the colonies.
|Fig 20. Gram-stained appearance of Fusobacterium|
nucleatum subsp. nucleatum, illustrating the fusiform
morphology of this organism.
|Fig 21. RapID ANA-II preformed enzyme system. One of many systems available for|
rapid definitive identification of commonly isolated
|Fig 22. Identification of Clostridium spp. Use this chart for organisms fulfilling|
the following three criteria: (1) anaerobic, (2) gram-positive bacilli, and (3) spore formers.
|Fig 23. Appearance of Actinomyces israelii showing|
the “molar tooth” colonies typical for this anaerobe.
|Fig 24. Gram-stained appearance of|
Propionibacterium acnes, illustrating the term “diphtheroid.”
|Fig 25. Gram-stained appearance of Bacteroides|
thetaiotaomicron, illustrating the typical appearance of
|Fig 26. Gram-stained appearance of Fusobacterium|
mortiferum, illustrating pleomorphism.
|Fig 28. Gram-stained appearance of a|
Peptostreptococcus sp. illustrating the chain formation that
occurs with some species of anaerobic gram-positive cocci.
This is only a part of the book : Textbook of Diagnostic Microbiology 4th edition 2011 of authors: Connie R. Mahon, Donald C. Lehman and George Manuselis. If you want to view the full content of the book and support author. Please buy it here: https://goo.gl/IawVC1