Mycoplasma and Ureaplasma, Atlas of Mycoplasma and Ureaplasma, atlas in medical, tuyenlab,net, Textbook of Diagnostic Microbiology 4th edition 2011, Connie R. Mahon, Donald C. Lehman and George Manuselis
|Fig 1. Typical large Mycoplasma colony showing|
“fried egg” appearance.
|Fig 2. Electron micrographs showing effect of Mycoplasma pneumoniae on|
ciliated tracheal cells. A, Infected animal model. B, Uninfected animal model.
|Fig 3. Electron micrograph of Mycoplasma|
pneumoniae attaching by specific attachment features to
ciliated trachea. mv, Microvilli; m, mycoplasma; c, cilia.
|Fig 4. Typical chest radiograph of a patient with a|
3-week course of atypical pneumonia. Note nonspecific
interstitial pneumonia and patchy infiltrate delineated by
†Thin colony periphery. Examine with Stereomicroscope using 20× to 60× magnification.
‡Color change: positive, yellow color with no gross turbidity; negative, red color.
§GP–RBC–HAD = Guinea pig red blood cell hemadsorption. b-Hemolysis test for presumptive identification of Mycoplasma pneumoniae may be used in lieu of GP–RBC–HAD.
NOTE: Methylene blue on Dienes stain can be used for detection of Mycoplasma spp. on SP4 agar; plate immunofluorescence using labeled antibody can be used for identification.
Fig 5. Flow diagram for Mycoplasma spp. isolation using classic methods.
|Fig 6. Dienes stain of Mycoplasma spp. colonies|
demonstrating typical “fried-egg” appearance.
|Fig 7. Typical mixed sizes of Mycoplasma spp. on|
primary isolation media: Mycoplasma salivarium.
Mixed isolation of Mycoplasma hominis andUreaplasma urealyticum showing why U. urealyticum was|
originally called “T” for “tiny-strain” (arrow).
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